Journal: bioRxiv

Article Title: Role of the high-affinity leukotriene B 4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice

doi: 10.1101/390914

Figure Lengend Snippet: A) Photomicrographs showing H&E-stained transverse sections of WT kidney. Scale bars = 500 μm. B) Changes in thickness of the WT UUO kidney cortex. Data are expressed as the mean ± SEM (n=4 mice/group). **P<0.01, vs . sham. C) Typical images showing Sirius red staining of WT kidney cortex after UUO. Scale bars = 200 μm. D) Soluble collagen in the WT UUO kidney cortex measured in the Sircol collagen assay. Data are expressed as the mean ± SEM (n=6 mice/group). **P<0.01, vs . Day 0. E,F) Expression of mRNA encoding BLT1 (E) and 5-LOX (F) in UUO kidneys, as measured by real-time RT-PCR. Data are expressed as the mean ± SEM (n=8-15 mice per group). *P<0.05 and **P<0.01, vs . WT; +P<0.05 and ++ P<0.01, vs . Day 0 WT. G) Images of sections of WT and BLT1 -/- UUO kidney cortex immunostained with an anti-5-LOX antibody on Days 0 and 3. Epithelial cells in dilated renal tubules and some interstitial cells stained positive for 5-LOX. Scale bars = 200 μm.

Article Snippet: Periodate-lysine-paraformaldehyde tissues were fixed for 2 h at 4°C, frozen in liquid nitrogen, cut into 10 μm sections, and stained as described above with one of the following primary antibodies: anti-5-LOX (1:100 dilution; Novus Biologicals, Littleton, CO, USA; NB 100-92138), anti-CXCL12 (1:100 dilution; eBioscience, San Diego, CA, USA; 14-7992), or anti-F4/80 (1:200 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA; sc-52664).

Techniques: Staining, Sircol Collagen Assay, Expressing, Quantitative RT-PCR

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Microsomal glutathione transferase 1 confers cisplatin resistance of non-small cell lung cancer via interaction with arachidonate lipoxygenase 5 to repress ferroptosis

doi: 10.22038/ijbms.2024.79203.17160

Figure Lengend Snippet: MGST1 was up-regulated, but ALOX5 was down-regulated in DDP-resistant NSCLC samples and cells

Article Snippet: The membranes were blocked with 5% skim milk for one hour and then incubated with primary antibodies against MGST1 (PA5-79670, 1:1000, Thermo Fisher), ALOX5 (#3289, 1:1000, CST, USA), glutathione peroxidase4 ( GPX4, A21440, 1:500, ABclonal, Wuhan, China), solute carrier family 7 member 11 (SLC7A11, A2413, 1:500, ABclonal), Acyl-CoA synthetase (ACSL4, A20414, 1:1000, ABclonal), and reduced glyceraldehyde-phosphate dehydrogenase (GAPDH, A19056, 1:10000, ABclonal) overnight at 4 ° C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (AS014, 1:2000, ABclonal) for one hour.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Microsomal glutathione transferase 1 confers cisplatin resistance of non-small cell lung cancer via interaction with arachidonate lipoxygenase 5 to repress ferroptosis

doi: 10.22038/ijbms.2024.79203.17160

Figure Lengend Snippet: MGST1 led to ferroptosis inhibition in NSCLC cells via direct interaction with ALOX5

Article Snippet: The membranes were blocked with 5% skim milk for one hour and then incubated with primary antibodies against MGST1 (PA5-79670, 1:1000, Thermo Fisher), ALOX5 (#3289, 1:1000, CST, USA), glutathione peroxidase4 ( GPX4, A21440, 1:500, ABclonal, Wuhan, China), solute carrier family 7 member 11 (SLC7A11, A2413, 1:500, ABclonal), Acyl-CoA synthetase (ACSL4, A20414, 1:1000, ABclonal), and reduced glyceraldehyde-phosphate dehydrogenase (GAPDH, A19056, 1:10000, ABclonal) overnight at 4 ° C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (AS014, 1:2000, ABclonal) for one hour.

Techniques: Inhibition

Journal: Immunity

Article Title: Tuft cell-derived leukotrienes drive rapid anti-helminth immunity in the small intestine but are dispensable for anti-protist immunity

doi: 10.1016/j.immuni.2020.02.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse 5-LO staining was performed as for DCLK1 above, with following modifications: 1 h 10% goat serum at room temperature (RT), overnight rabbit anti-5-LO (Sigma Aldrich, SAB1410449) at 4° C, 40 min goat anti-rabbit IgG F(ab’)2-AF594, 1 h 10% rabbit serum at RT, 2 h αDCLK1-AF488 (custom conjugation of ab31704) at RT.

Techniques: Recombinant, Plasmid Preparation, Blocking Assay, Staining, Enzyme-linked Immunosorbent Assay, Software